The ability of inner-cell-mass cells to self-renew as embryonic stem cells is acquired following epiblast specification

Boroviak T., Loos R., Bertone P., Smith A. and Nichols J.Nature Cell Biology, 2014

The authors show that mouse ICM cells from early blastocysts can progress to ERK independence if provided with a specific laminin substrate. We show by RNA sequencing that Laminin-511 is expressed in the early ICM, as is B1-integrin, which mediates laminin binding in ESCs. No expression of Laminin-521 in mouse ICM. Single ESC derivation in 2i-LIF on a fibronectin-LN511 mix and clonal ESC lines expansion on LN511.