Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors

Sellgren C.M., Sheridan S.D., Gracias J., Xuan D., Fu T., Perlis R.H.Molecular Psychiatry, 2017

Human fibroblasts were reprogrammed and the resulting iPSC colonies stabilized and expanded under xeno-free conditions by Stemiotics. Colonies were bulk passaged from the most productive well to establish passage 1 (P1) iPSC cultures on Laminin-521 in Nutristem XF media and expanded in the same culture system until at least passage 3 before being frozen down for storage. The iPSC were then used for neural differentiation.