Development of a biomaterial associated with mesenchymal stem cells and keratinocytes for use as a skin substitute

Steffens D., Mathor M.B., Santi B.T.S., Luco D.P., Pranke P.Regen. Med., 2015

Here they developed s scaffolds of poly-DL-lactic acid with and without the linkage of laminin-332, bringing together MSCs and keratinocytes aimed for treatment as a new skin substitute. Three groups of scaffolds were studied: 1) poly-DL-lactic acid (PDLLA), 2) hydrolyzed PDLLA (PDLLA/NaOH) and 3) PDLLA/Lam which is a PDLLA/NaOH scaffold linked to laminin-332. The results corroborate the hypothesis that laminin influenced the adhesion of the MSCs. Laminin significantly promoted the adhesion and spreading of proliferating oral and epidermal keratinocytes compared with collagen nanofibers only. The use of biocompatible and biodegradable polymers associated with the properties of laminin leads to an improvement in the adherence and viability of the cells, showing LN-332 is beneficial for the growth of MSCs and keratinocytes.