CCR2+CCR5+ T Cells Producing Matrix Metalloproteinase-9 and Osteopontin in the Pathogenesis of Multiple Sclerosis

Sato W., Tomita A., Ichikawa D., Lin Y., Kishida H., Miyake S., Ogawa M., Okamoto T., Murata M., Kuroiwa Y., Aranami T., Yamamura T.
Journal of Immunology, 2012

The authors coated the upper sides of Transwell membrane inserts with Biolaminin 211. The aim was to recapitulate the glia limitans layered with parenchymal basal lamina experimentally, to study how effectively T-cells invade accross the physiological barrier separating CSF from the CNS parenchyma. Normal human astrocytes were seeded on the lower sides of the membrane inserts. T-cell migration across the astrocyte layered with laminin was less efficient and thus, this model would exhibit barrier functions against the penetration of activated T cells.